questions about count output

hqyone / tRNAExplorer

A small RNA-Seq NGS pipeline to analysis tRNA pool in Cells

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questions about count output #3

Open lkong888 opened 8 months ago

lkong888 commented 8 months ago

Hi,

Great tools to explore the tRF comprehensively. May I ask why all the count values are float instead of int? Are they normalized read count? (for example: trna_sample_readcount_matrix.tsv). Thanks!

hqyone commented 4 months ago

Hi, this is because there are a lot of multiple aligned reads, we have to split them based on unique aligned reads.