Hello BRIE2,
Thanks for this amazing package!
I'm using the loom file as input, but it generates errors as below. Also tried on py3.7, doesn't work.
Environment: Python 3.8, Ubuntu 20.04 LTS
Could you please help me with this issue?
Thanks!
Best,
YJ
(base) hyjforesight@W10D-GW97ZC3:~$ brie-quant -i /home/hyjforesight/loom/cellsorted_WT_IEC_G123B.loom -o /home/hyjforesight/brie2/brie_quant_cell.h5ad -c /home/hyjforesight/WT_IEC_barcodes.tsv --interceptMode=gene --LRTindex=All --layers spliced,unspliced
2021-11-30 18:49:53.274751: W tensorflow/stream_executor/platform/default/dso_loader.cc:64] Could not load dynamic library 'libcudart.so.11.0'; dlerror: libcudart.so.11.0: cannot open shared object file: No such file or directory
2021-11-30 18:49:53.275065: I tensorflow/stream_executor/cuda/cudart_stub.cc:29] Ignore above cudart dlerror if you do not have a GPU set up on your machine.
Traceback (most recent call last):
File "/home/hyjforesight/miniconda3/bin/brie-quant", line 8, in
sys.exit(main())
File "/home/hyjforesight/miniconda3/lib/python3.8/site-packages/brie/bin/quant.py", line 213, in main
quant(options.in_file, options.cell_file, options.gene_file,
File "/home/hyjforesight/miniconda3/lib/python3.8/site-packages/brie/bin/quant.py", line 53, in quant
_idx = brie.match(adata.obs.index, dat_tmp[1:, 0]).astype(float)
UnboundLocalError: local variable 'adata' referenced before assignment
Hello BRIE2, Thanks for this amazing package! I'm using the loom file as input, but it generates errors as below. Also tried on py3.7, doesn't work. Environment: Python 3.8, Ubuntu 20.04 LTS Could you please help me with this issue? Thanks! Best, YJ
(base) hyjforesight@W10D-GW97ZC3:~$ brie-quant -i /home/hyjforesight/loom/cellsorted_WT_IEC_G123B.loom -o /home/hyjforesight/brie2/brie_quant_cell.h5ad -c /home/hyjforesight/WT_IEC_barcodes.tsv --interceptMode=gene --LRTindex=All --layers spliced,unspliced 2021-11-30 18:49:53.274751: W tensorflow/stream_executor/platform/default/dso_loader.cc:64] Could not load dynamic library 'libcudart.so.11.0'; dlerror: libcudart.so.11.0: cannot open shared object file: No such file or directory 2021-11-30 18:49:53.275065: I tensorflow/stream_executor/cuda/cudart_stub.cc:29] Ignore above cudart dlerror if you do not have a GPU set up on your machine. Traceback (most recent call last): File "/home/hyjforesight/miniconda3/bin/brie-quant", line 8, in
sys.exit(main())
File "/home/hyjforesight/miniconda3/lib/python3.8/site-packages/brie/bin/quant.py", line 213, in main
quant(options.in_file, options.cell_file, options.gene_file,
File "/home/hyjforesight/miniconda3/lib/python3.8/site-packages/brie/bin/quant.py", line 53, in quant
_idx = brie.match(adata.obs.index, dat_tmp[1:, 0]).astype(float)
UnboundLocalError: local variable 'adata' referenced before assignment