creat database

[huichen5796]

• [x] create local database
• [x] deploy the database to Cloud
  • Amazon Web Services (AWS)
  • Amazon API Gateway

[huichen5796]

Zellen

• Sample ID
• RunDate
• Machine version
• Viability
  • Viability (%)
  • Total cells / ml (x 10^6)
  • Total viable cells / ml (x 10^6)
• Morphology
  • Average diameter (microns)
  • Average circularity
  • Cell type
  • (acrosome and membrane integrity of sperm)
• Flow cytometry analysis
  • characteristic phenotype
  • mitochondrial activity
  • cell metabolic activities
  • DNA fragmentation
  • oxidative/antioxidative status markers (hydrogen peroxide [H2O2], superoxide dismutase [SOD], glutathione peroxidase [GPx], catalase, malondialdehyde [MDA], and total antioxidant capacity [TAC])
• Parameters during Cell Culture
  • proliferative activity
  • differentiation potential
  • early apoptotic behaviors
• Mobility
  • total mobility
  • progressive mobility

[huichen5796]

CPA

• Komponents
• pH
• osmolality
• FTIR curve
  • computing second derivative spectra
  • computing inverted second derivative spectra
  • Band ratios of characteristic molecular group vibrations obtained from the spectra
• DSC curve
• viscosity
• Cryomicroscopy ?

[huichen5796]

Process

• sample volume
• -cell konzentration
• cooling rate
• warming rate
• methode
  • Slow freezing
  • non-equilibrium vitrification
  • equilibrium vitrification
  • A new cryotop vial device system provides an aseptic cryoprotectant-free and centrifuge-free cryopreservation of human spermatozoa (a closed system)