hwc2021
commented
6 months ago Hi,
It seems that there is something wrong with the input files or SPAdes software, please make sure that all the input files are available. And the SPAdes software must be successfully installed in the PATH (Please see the Requirement section of the README.md).
Hi He, may I ask how to handle the following error when running the program Warning: Please note that the SPAdes is not recommended for large sequencing dataset > 5 gb. You can use a subset instead. Read1: fastp/FDES230025508-1r_L1_1.fq ; Read2:fastp/FDES230025508-1r_L1_2.fq python: invalid option -- '-' usage: python [-d] [-i] [-k] [-v] [-c cmd | file | -] [arg] ...
Options and arguments (and corresponding environment variables): -d : debug output from parser (also PYTHONDEBUG=x) -i : inspect interactively after running script (also PYTHONINSPECT=x) -k : kill printing expression statement (also PYTHONKILLPRINT=x) -v : verbose (trace import statements) (also PYTHONVERBOSE=x) -c cmd : program passed in as string (terminates option list) file : program read from script file
Other environment variables: PYTHONSTARTUP: file executed on interactive startup (no default) PYTHONPATH : colon-separated list of directories prefixed to the default module search path. The result is sys.path. Cannot open the file: 没有那个文件或目录