Open mattoslmp opened 4 years ago
Prodigal doesn't predict ORFs - it finds genes (which are substrings of ORFs). If you want ORFs, try orfm
.
It is not possible to change the MIN_GENE size via the command line. You need to edit the source code and recompile.
But 15bp is far too small, you will get 1000s of false positives. There are very few real genes that small. You could build a DB of the tiny ones and then tblastn
them to assign them.
Thank you for your help and explanation @tseemann.
@tseemann Hi!I would like to predict genes between 33 and 303 bp. How can I edit the source code and recompile? Thanks!
@KeyLllll Have you solved this issue?
How Can I change the length parameter of predicted ORFs. I would like to specify sequences > 15bp, predict sequences larger than this size.