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hyeshik / poreplex

A versatile sequenced read processor for nanopore direct RNA sequencing
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Unhandled exception KeyError: "Unable to open object (object 'Analyses' doesn't exist)" #26

Open amitblum opened 3 years ago

amitblum commented 3 years ago

Hello, I got the following error: Unhandled exception KeyError: "Unable to open object (object 'Analyses' doesn't exist)"

2020-10-06 16:07:12,361 Starting poreplex version 0.4.1 2020-10-06 16:07:12,361 Command line: /apps/RH7U2/gnu/python/3.5.2/bin/poreplex -i /home/labs/schwartzlab/amitblum/nanopre_rawdata/L821_4_BCs1_4/L821_BC1_4/BCs1_4/20200923_1453_MN34379_FAO02325_d4e35397/fast5/ -o /home/labs/schwartzlab/amitblum/nanopre_rawdata/L821_4_BCs1_4/fastq/ --barcoding --parallel 20 2020-10-06 16:07:12,361 == Analysis settings ====================================== 2020-10-06 16:07:12,361 Input: /home/labs/schwartzlab/amitblum/nanopre_rawdata/L821_4_BCs1_4/L821_BC1_4/BCs1_4/20200923_1453_MN34379_FAO02325_d4e35397/fast5/ 2020-10-06 16:07:12,361 Output: /home/labs/schwartzlab/amitblum/nanopre_rawdata/L821_4_BCs1_4/fastq/ 2020-10-06 16:07:12,361 Processes: 20 2020-10-06 16:07:12,361 Presets: rna-r941.cfg 2020-10-06 16:07:12,361 Basecall on-the-fly: No (use previous analyses) 2020-10-06 16:07:12,361 Trim 3' adapter: No 2020-10-06 16:07:12,361 Filter concatenated read: No 2020-10-06 16:07:12,361 Separate by barcode: Yes 2020-10-06 16:07:12,361 Real-time alignment: No 2020-10-06 16:07:12,362 FASTQ in output: Yes 2020-10-06 16:07:12,362 FAST5 in output: No 2020-10-06 16:07:12,362 Basecall table in output: No 2020-10-06 16:07:12,362 =========================================================== 2020-10-06 16:07:12,362 2020-10-06 16:24:33,811 [signal_analyzer.py:114] (FAO02325_50a12c2b_180.fast5#da6b9bc1-5f79-4689-bc43-d616855215b8) Unhandled exception KeyError: "Unable to open object (object 'Analyses' doesn't exist)"

Do you have any suggestion how to solve it?

Thanks Amit