nathaliaraquelx
commented
3 years ago Meeting on April 29th
Topics discussed:
- Detection of mini cells (flow cytometry, electron microscopy or DIC microscopy) 🥰
- Determination of the isolated mini cells characteristics (purification)
- Making sure mini cells can't divide (fluorescence microscopy in different periods of time)
- Separating mini cells from parental cells (ratio of mini cells to normal cells before and after purification; HPLC of mini cells(?)) 🤔
- Leakiness of mini cells (?) ðŸ’
- Ask donation of E. coli strain TB43 to be used as a positive control on mini cell characterization assays 💯
- Team iGEM Marburg 2018 has designed a sRNA-anti-MinC that may be useful for us to compare the ratio of mini cells production to our own design and use as a proof of concept of improvement of mini cell production
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In this issue, the members of team C (we can come up with a cooler name :)) will add their research, ideas...