Closed jakebeal closed 2 years ago
One could add standard stretches 5' and 3' that are cleaved during the type II assembly.
I'll reach out to Marc about what Freegenes normally does, we don't have to follow them though. But as an example, they have B0034 here https://freegenes.github.io/genes/BBF10K_003410.html where they have a 43 bp insert with 400bp additionally synthesized (60 of which is for their barcode)
I made some quick additions and deletions to their annotations, to show the extra bases that they added. Not great, just to be quick https://benchling.com/s/seq-b8FOT2bE7kCrp4z7pCeY. Couldn't attach a .gb file.
Should the padding sequences adjusted the minimum length requirement so we're not paying for extra unused bases what could represent a substantial price on the volume ? I would probably not go to an adjustment by the base but rather with couple sets of padding sequences with intermediate length that we know get synthesized nicely.
Closing this as #213 and #231 exist
Twist has a minimum synthesis length that is longer than many promoters and terminators (150 bp, iirc). How should we approach padding for short sequences?