gRNA expression device for Cas

[vinoo-igem]

As we consider what the must-haves and nice-to-haves are for a CRISPR-Cas kit: should we be providing an expression device/plasmid for gRNA paired with a particular Cas? This may all be moot if we want to encourage teams to assemble these as two separate devices instead, and then a MTU.

I imagine there are many considerations here, but to get the ball rolling.

• Cloning method
• Cas selection
• Plasmid selection
• Target organism

[jakebeal]

If we add Cas repressor devices (e.g., https://doi.org/10.1038/ncomms15459, https://www.nature.com/articles/nmeth.2969), when we would definitely want to do this. In this case, the device actually consists of three parts: the Cas, the gRNA expression region, and the targeted promoter.

With Cas used for editing, are there "favorite" sites that people target?

[ethanj801]

With Cas used for editing, are there "favorite" sites that people target?

I know David Liu's lab typically uses HEK3, DNMT1, RNF2, EMX1, FANCF, RUNX1 and VEGFA to test the efficiency of their prime and base editing variants in human cells.