search

iMetOsaka / UNAGI

3 stars 4 forks source link

Could the pipeline be used to the data of nanopore direct RNA sequencing? #1

Closed q1134269149 closed 3 years ago

q1134269149 commented 4 years ago

Hi, Thank you very much for providing us with this pipeline to process nanopore data. I read your research paper, and the data of nanopore full-length cdna sequencing was used to analyse. Because I got my data with nanopore direct RNA sequencing and the sequence of bases in these reads extends from 3' to 5' which is backward to the cdna sequencing, could the pipeline be used to my data of nanopore direct RNA sequencing? Looking forward to your reply. Thanks hqin

imet-k commented 4 years ago

Thank you for your interest in our pipeline and very sorry for the late response. In my experience, the base-caller (Guppy) will produce the sequences in fastq file in the right direction 5' to 3' and thus our pipeline is totally compatible with DRS. Please let us know if you have any problems.

Kind Regards,

JungNicolas commented 3 years ago

I'm closing this issue since it seems solved for the moment.