some problems of input data

icebert / eccDNA_RCA_nanopore

eccDNA identification from nanopore long reads of rolling-circle amplicon

MIT License

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some problems of input data #4

Open lemontin opened 2 years ago

lemontin commented 2 years ago

Hi meng, I'm wondering why trim adapter with porechop after Guppy trim barcode? BC and adapter has removed after guppy.

icebert commented 2 years ago

Based on the description of Guppy, it should trim away adaptors. However, when I examined the fastqs generated by Guppy, I can still find many reads containing adaptors. So I have to use porechop to trim the adaptors separately.