Open GoogleCodeExporter opened 9 years ago
Could you try viewing the error stream in the terminal using the error log path
/usr/pl_cache/pipeline/.../SOAPtoSAM_1_1.error at the bottom?
Sam
Original comment by shobe...@gmail.com
on 8 Aug 2011 at 10:58
The error is this one:
/projects1/idinov/projects/Pipeline_genomics_informatics_2011/scripts/SOAP/soap2
sam.sh: line 6: /ifshome/shobel/Bioinformatics/soap2.21/soap2sam.pl: No such
file or directory
I checked in the script. Actually I noticed that the path to the .pl is missing
(there was the one on cranium)...I can find the soap2sam.sh in
/projects1/idinov/projects/Pipeline_genomics_informatics_2011/scripts/SOAP/,
but not the .pl that is evoked in the .sh script.
DO you think that my interpretation of this error is correct?
Federica
Original comment by federica...@gmail.com
on 8 Aug 2011 at 11:24
Yes this is correct. The path called in the script is the path on cranium and
needs to be changed to the path on fgene. But first we need to copy soap2sam.pl
over to your server and the problem will be fixed.
A similar problem might arise in the BWA workflow if the script wasn't changed,
but you won't receive an error. Do you know if the paths in the bwa_sam.sh
script were changed from /ifshome/shobel/Bioinformatics/bwa-0.5.9rc1/bwa to
your server?
Sam
Original comment by shobe...@gmail.com
on 9 Aug 2011 at 7:03
(1) ok, let me know when you have copied the .pl so I can test the workflow.
(2) I am actuallyusing this script:
/projects1/test_pipeline/NEW_ORGANIZATION/scripts/bwa_sam.sh. I have modified
it. i corrected one space and now I am runnign again the BWA PE and SE. Will
let you know.
Fed
Original comment by federica...@gmail.com
on 9 Aug 2011 at 7:16
Ivo says he has copied over soap2sam.pl so it should be in
/projects1/idinov/projects/Pipeline_genomics_informatics_2011/scripts/SOAP/.
I don't have access to the modified bwa script you are using but it sounds like
you know what you are doing. It got a bit messy trying to run single or paired
reads along with an unknown number of options because the reads are being fed
from the previous module instead of a data source. If you have any questions
about it, let me know.
Sam
Original comment by shobe...@gmail.com
on 9 Aug 2011 at 8:12
Hi,
here the folder:
fgene3
[/projects1/idinov/projects/Pipeline_genomics_informatics_2011/scripts/SOAP] ll
total 116
-rwxr-xr-x 1 idinov genetics 134 May 2 19:36 Queue.sh
-rwxr-xr-x 1 idinov genetics 252 Jun 16 15:40 Rwrapper.sh
-rwxr-xr-x 1 idinov genetics 511 Jun 7 16:26 Rwrapper_ifs.sh
-rwxr-xr-x 1 idinov genetics 143 May 3 16:22 ValidateSam.sh
-rwxr-xr-x 1 idinov genetics 278 Jul 19 16:40 buildstring
-rwxr-xr-x 1 idinov genetics 85 Jul 21 15:15 bwa_aln.sh
-rwxr-xr-x 1 idinov genetics 1382 Aug 5 13:21 bwa_sam.sh
-rwxr-xr-x 1 idinov genetics 137 May 12 15:58 bwaindex.sh
-rwxr-xr-x 1 idinov genetics 378 Jun 5 19:37 compliments.sh
-rwxr-xr-x 1 idinov genetics 371 Mar 29 17:37 exercise2.1.sh
-rwxr-xr-x 1 idinov genetics 627 May 6 23:25 gammaconfig.sh
-rwxr-xr-x 1 idinov genetics 456 May 26 18:53 gammacoreel.sh
-rwxr-xr-x 1 idinov genetics 385 May 26 18:11 gammacoregamma.sh
-rwxr-xr-x 1 idinov genetics 338 May 7 00:05 gammacorelatent.sh
-rwxr-xr-x 1 idinov genetics 318 May 26 18:20 gammacorelatent2.sh
-rwxr-xr-x 1 idinov genetics 318 May 26 18:35 gammacorelatent3.sh
-rwxr-xr-x 1 idinov genetics 767 May 6 21:48 gammafilelist.sh
-rwxr-xr-x 1 idinov genetics 175 Apr 13 17:31 indexing.sh
-rwxr-xr-x 1 idinov genetics 94 May 26 17:47 makecopy.sh
-rwxr-xr-x 1 idinov genetics 1249 Jul 21 13:44 maq_mapper.sh
-rwxr-xr-x 1 idinov genetics 202 Apr 22 15:35 migrate.sh
-rwxr-xr-x 1 idinov genetics 191 Jul 29 14:56 mosaikbuild.sh
-rwxr-xr-x 1 idinov genetics 207 Jul 29 14:59 mosaikbuild2.sh
-rwxr-xr-x 1 idinov genetics 302 Apr 28 13:01 msanalyser.sh
-rwxr-xr-x 1 idinov genetics 175 Jul 19 15:13 parameters.sh
-rwxr-xr-x 1 idinov genetics 118 Jun 27 16:58 soap2sam.sh
-rwxr-xr-x 1 idinov genetics 206 May 9 16:12 sortsam.sh
-rwxr-xr-x 1 idinov genetics 432 Jun 17 14:24 upload.sh
-rwxr-xr-x 1 idinov genetics 544 May 20 19:03 uploadQA.sh
..I don't see the perl. I see the .sh.
federica
Original comment by federica...@gmail.com
on 9 Aug 2011 at 9:41
Hi Federica - my bad (I confused the soap2sam.pl and soap2sam.sh files). Now
both are here:
/projects1/idinov/projects/Pipeline_genomics_informatics_2011/scripts/SOAP
_ Ivo
Original comment by iwaterp...@gmail.com
on 10 Aug 2011 at 6:35
Perfect I am running both SE and PE already.
thanks!
Original comment by federica...@gmail.com
on 10 Aug 2011 at 6:52
PERFECT! SUCCESSFUL RUN! Other two modules added in the library... :)
Original comment by federica...@gmail.com
on 10 Aug 2011 at 10:07
Original issue reported on code.google.com by
federica...@gmail.com
on 3 Aug 2011 at 10:56Attachments: