Combining overlapping ROIs which include a non-primitive shape produces an inaccurate area measure. I would expect a fully overlapping ROI to have no effect on the area measure of the enclosing ROI.
Note that in my testing a freehand selection appears larger after combining with a fully overlapping oval, while a segmented ROI (as above) appears smaller.
From: http://forum.imagej.net/t/sum-of-areas-with-the-command-or-combine/11467/3
Combining overlapping ROIs which include a non-primitive shape produces an inaccurate area measure. I would expect a fully overlapping ROI to have no effect on the area measure of the enclosing ROI.
Steps to reproduce (IJ1 code):
Note that in my testing a freehand selection appears larger after combining with a fully overlapping oval, while a segmented ROI (as above) appears smaller.