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immcantation / presto

pRESTO is part of the Immcantation analysis framework for Adaptive Immune Receptor Repertoire sequencing (AIRR-seq). pRESTO is a bioinformatics toolkit for processing high-throughput lymphocyte receptor sequencing data.
https://presto.readthedocs.io
GNU Affero General Public License v3.0
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Add example workflow for UMI following primer #69

Open ssnn-airr opened 5 years ago

ssnn-airr commented 5 years ago

Original report by Jason Vander Heiden (Bitbucket: javh, GitHub: javh).

We could use either a small example or a full workflow for data where the UMI is after the primer region (using MaskPrimers-extract).

Example protocol:

Turchaninova, M. A. et al. High-quality full-length immunoglobulin profiling with unique molecular barcoding. Nat. Protoc. 11, 1599–1616 (2016).

Though, that might just be a case of using the TS site as the primer: CTTGGGG