Closed hbandukw closed 2 years ago
Hi there,
Apologies for the delay in getting back to you. We currently do not have the ability to generate a Symphony reference using only the harmonized embedding, unfortunately. This is because the Symphony soft cluster centroids and "reference compression terms" are not saved into the Seurat object by default, and we currently don't have a way to back-calculate them after the fact. If you have the list of steps and parameters that were used to generate the Seurat object, I wonder if you can recreate it, then use the RunHarmony.Seurat
function in vignettes/utils_seurat.R
(following our Seurat tutorial).
Hello,
I have an atlas that was produced by integrating data with Harmony. I want to use the integrated data with Symphony's mapQuery function. The recommended option i.e. Option 1 listed on https://github.com/immunogenomics/symphony starts with an existing harmony object which is then used to make a Symphony reference using buildReferenceFromHarmonyObj.
I currently have the harmonized embeddings integrated into a .h5seurat file. Is there a way I can extract the necessary components from this file and load them into R as a harmony object?
Please note that I have also posted this issue on harmony: https://github.com/immunogenomics/harmony/issues/142