Open amarinderthind opened 2 years ago
Could you please send me the session info and the full code?
Do you have access to internet ?
Thank you,
Lucas A Salas
On Aug 4, 2022, at 9:54 PM, Amarinder @.***> wrote:
Hi Author, I am getting the following error with the function libraryDataGet
snapshotDate(): 2022-04-26 snapshotDate(): 2022-04-26 see ?FlowSorted.Blood.EPIC and browseVignettes('FlowSorted.Blood.EPIC') for documentation loading from cache Error: failed to load resource name: EH1136 title: FlowSorted.Blood.EPIC: Illumina Human Methylation data from EPIC on immunomagnetic sorted adult blood cell populations reason: error in evaluating the argument 'x' in selecting a method for function 'get': empty (zero-byte) input file
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The Internet is working fine on a Linux machine...
library(FlowSorted.Blood.EPIC) ##sucessful
FlowSorted.Blood.EPIC <- libraryDataGet("FlowSorted.Blood.EPIC") cellCounts <- estimateCellCounts2(rgSet, referencePlatform ="IlluminaHumanMethylationEPIC")
sessionInfo(package = 'FlowSorted.Blood.EPIC' ) R version 4.2.0 (2022-04-22) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 18.04.6 LTS
Matrix products: default BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.7.1 LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.7.1
locale:
[1] LC_CTYPE=en_AU.UTF-8 LC_NUMERIC=C LC_TIME=en_AU.UTF-8 LC_COLLATE=en_AU.UTF-8
[5] LC_MONETARY=en_AU.UTF-8 LC_MESSAGES=en_AU.UTF-8 LC_PAPER=en_AU.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_AU.UTF-8 LC_IDENTIFICATION=C
attached base packages: character(0)
other attached packages: [1] FlowSorted.Blood.EPIC_2.0.0
loaded via a namespace (and not attached):
[1] utf8_1.2.2 R.utils_2.12.0
[3] tidyselect_1.1.2 RSQLite_2.2.14
[5] AnnotationDbi_1.58.0 htmlwidgets_1.5.4
[7] grid_4.2.0 BiocParallel_1.30.3
[9] devtools_2.4.4 munsell_0.5.0
[11] codetools_0.2-18 preprocessCore_1.58.0
[13] interp_1.1-3 statmod_1.4.36
[15] miniUI_0.1.1.1 withr_2.5.0
[17] colorspace_2.0-3 Biobase_2.56.0
[19] filelock_1.0.2 IlluminaHumanMethylationEPICanno.ilm10b5.hg38_0.0.1
[21] knitr_1.39 rstudioapi_0.13
[23] stats4_4.2.0 MatrixGenerics_1.8.1
[25] FlowSorted.Blood.450k_1.34.0 GenomeInfoDbData_1.2.8
[27] stats_4.2.0 bit64_4.0.5
[29] rhdf5_2.40.0 vctrs_0.4.1
[31] generics_0.1.2 xfun_0.31
[33] biovizBase_1.44.0 BiocFileCache_2.4.0
[35] R6_2.5.1 GenomeInfoDb_1.32.2
[37] illuminaio_0.38.0 locfit_1.5-9.6
[39] AnnotationFilter_1.20.0 bitops_1.0-7
[41] rhdf5filters_1.8.0 cachem_1.0.6
[43] reshape_0.8.9 DelayedArray_0.22.0
[45] assertthat_0.2.1 promises_1.2.0.1
[47] BiocIO_1.6.0 IlluminaHumanMethylation450kanno.ilmn12.hg19_0.6.1
[49] scales_1.2.0 bsseq_1.32.0
[51] nnet_7.3-17 gtable_0.3.0
[53] processx_3.7.0 ensembldb_2.20.2
[55] rlang_1.0.4 utils_4.2.0
[57] methods_4.2.0 genefilter_1.78.0
[59] splines_4.2.0 rtracklayer_1.56.1
[61] lazyeval_0.2.2 DSS_2.44.0
[63] GEOquery_2.64.2 dichromat_2.0-0.1
[65] checkmate_2.1.0 BiocManager_1.30.18
[67] yaml_2.3.5 GenomicFeatures_1.48.3
[69] backports_1.4.1 httpuv_1.6.5
[71] base_4.2.0 Hmisc_4.7-0
[73] usethis_2.1.6 tools_4.2.0
[75] nor1mix_1.3-0 ggplot2_3.3.6
[77] ellipsis_0.3.2 RColorBrewer_1.1-3
[79] BiocGenerics_0.42.0 siggenes_1.70.0
[81] sessioninfo_1.2.2 Rcpp_1.0.8.3
[83] plyr_1.8.7 base64enc_0.1-3
[85] sparseMatrixStats_1.8.0 progress_1.2.2
[87] zlibbioc_1.42.0 purrr_0.3.4
[89] RCurl_1.98-1.7 ps_1.7.1
[91] prettyunits_1.1.1 IlluminaHumanMethylationEPICmanifest_1.0.0
[93] rpart_4.1.16 openssl_2.0.2
[95] deldir_1.0-6 urlchecker_1.0.1
[97] bumphunter_1.38.0 S4Vectors_0.34.0
[99] SummarizedExperiment_1.26.1 cluster_2.1.3
[101] fs_1.5.2 magrittr_2.0.3
[103] data.table_1.14.2 ProtGenerics_1.28.0
[105] IlluminaHumanMethylationEPICanno.ilm10b4.hg19_0.6.0 matrixStats_0.62.0
[107] pkgload_1.3.0 missMethyl_1.30.0
[109] mime_0.12 hms_1.1.1
[111] xtable_1.8-4 XML_3.99-0.10
[113] jpeg_0.1-9 mclust_5.4.10
[115] IRanges_2.30.0 gridExtra_2.3
[117] compiler_4.2.0 biomaRt_2.52.0
[119] minfi_1.42.0 tibble_3.1.7
[121] crayon_1.5.1 R.oo_1.25.0
[123] htmltools_0.5.2 later_1.3.0
[125] tzdb_0.3.0 Formula_1.2-4
[127] tidyr_1.2.0 DBI_1.1.3
[129] ExperimentHub_2.4.0 dbplyr_2.2.1
[131] MASS_7.3-57 DMRcate_2.10.0
[133] rappdirs_0.3.3 Matrix_1.4-1
[135] readr_2.1.2 permute_0.9-7
[137] cli_3.3.0 quadprog_1.5-8
[139] datasets_4.2.0 grDevices_4.2.0
[141] R.methodsS3_1.8.2 parallel_4.2.0
[143] Gviz_1.40.1 GenomicRanges_1.48.0
[145] pkgconfig_2.0.3 GenomicAlignments_1.32.1
[147] IlluminaHumanMethylation450kmanifest_0.4.0 foreign_0.8-82
[149] xml2_1.3.3 foreach_1.5.2
[151] annotate_1.74.0 rngtools_1.5.2
[153] multtest_2.52.0 beanplot_1.3.1
[155] XVector_0.36.0 doRNG_1.8.2
[157] scrime_1.3.5 graphics_4.2.0
[159] callr_3.7.1 stringr_1.4.0
[161] VariantAnnotation_1.42.1 digest_0.6.29
[163] Biostrings_2.64.0 base64_2.0
[165] htmlTable_2.4.1 edgeR_3.38.1
[167] DelayedMatrixStats_1.18.0 restfulr_0.0.15
[169] curl_4.3.2 shiny_1.7.1
[171] Rsamtools_2.12.0 gtools_3.9.2.2
[173] rjson_0.2.21 lifecycle_1.0.1
[175] nlme_3.1-157 Rhdf5lib_1.18.2
[177] askpass_1.1 limma_3.52.2
[179] BSgenome_1.64.0 fansi_1.0.3
[181] pillar_1.7.0 lattice_0.20-45
[183] pkgbuild_1.3.1 KEGGREST_1.36.2
[185] fastmap_1.1.0 httr_1.4.3
[187] survival_3.2-13 remotes_2.4.2
[189] interactiveDisplayBase_1.34.0 glue_1.6.2
[191] png_0.1-7 iterators_1.0.14
[193] BiocVersion_3.15.2 bit_4.0.4
[195] profvis_0.3.7 stringi_1.7.6
[197] HDF5Array_1.24.1 blob_1.2.3
[199] AnnotationHub_3.4.0 org.Hs.eg.db_3.15.0
[201] latticeExtra_0.6-30 memoise_2.0.1
[203] dplyr_1.0.9
@Amarinder
I am trying a similar environment, the same versions of the libraries, and the function is working. Also, the test on Bioconductor is not providing me any errors for this function in the testthat.
I wonder if this is related to the ExperimentHub setup in your machine.
Could you try this?
library(ExperimentHub) tmp<-ExperimentHub()[["EH1136"]]
Does that work for you?
If it is not, could you check the options on ?ExperimentHub related to your cache and proxy?
I believe this could be the problem and not the library. But please let me know.
Best,
Lucas
From: Amarinder @.> Sent: Thursday, August 4, 2022 11:36 PM To: immunomethylomics/FlowSorted.Blood.EPIC @.> Cc: Lucas A. Salas @.>; Comment @.> Subject: Re: [immunomethylomics/FlowSorted.Blood.EPIC] Error failed to load with libraryDataGet (Issue #9)
library(FlowSorted.Blood.EPIC) ##sucessful
FlowSorted.Blood.EPIC <- libraryDataGet("FlowSorted.Blood.EPIC") cellCounts <- estimateCellCounts2(rgSet, referencePlatform ="IlluminaHumanMethylationEPIC")
sessionInfo(package = 'FlowSorted.Blood.EPIC' ) R version 4.2.0 (2022-04-22) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 18.04.6 LTS
Matrix products: default BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.7.1 LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.7.1
locale: [1] LC_CTYPE=en_AU.UTF-8 LC_NUMERIC=C LC_TIME=en_AU.UTF-8 LC_COLLATE=en_AU.UTF-8 [5] LC_MONETARY=en_AU.UTF-8 LC_MESSAGES=en_AU.UTF-8 LC_PAPER=en_AU.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_AU.UTF-8 LC_IDENTIFICATION=C
attached base packages: character(0)
other attached packages: [1] FlowSorted.Blood.EPIC_2.0.0
loaded via a namespace (and not attached): [1] utf8_1.2.2 R.utils_2.12.0 [3] tidyselect_1.1.2 RSQLite_2.2.14 [5] AnnotationDbi_1.58.0 htmlwidgets_1.5.4 [7] grid_4.2.0 BiocParallel_1.30.3 [9] devtools_2.4.4 munsell_0.5.0 [11] codetools_0.2-18 preprocessCore_1.58.0 [13] interp_1.1-3 statmod_1.4.36 [15] miniUI_0.1.1.1 withr_2.5.0 [17] colorspace_2.0-3 Biobase_2.56.0 [19] filelock_1.0.2 IlluminaHumanMethylationEPICanno.ilm10b5.hg38_0.0.1 [21] knitr_1.39 rstudioapi_0.13 [23] stats4_4.2.0 MatrixGenerics_1.8.1 [25] FlowSorted.Blood.450k_1.34.0 GenomeInfoDbData_1.2.8 [27] stats_4.2.0 bit64_4.0.5 [29] rhdf5_2.40.0 vctrs_0.4.1 [31] generics_0.1.2 xfun_0.31 [33] biovizBase_1.44.0 BiocFileCache_2.4.0 [35] R6_2.5.1 GenomeInfoDb_1.32.2 [37] illuminaio_0.38.0 locfit_1.5-9.6 [39] AnnotationFilter_1.20.0 bitops_1.0-7 [41] rhdf5filters_1.8.0 cachem_1.0.6 [43] reshape_0.8.9 DelayedArray_0.22.0 [45] assertthat_0.2.1 promises_1.2.0.1 [47] BiocIO_1.6.0 IlluminaHumanMethylation450kanno.ilmn12.hg19_0.6.1 [49] scales_1.2.0 bsseq_1.32.0 [51] nnet_7.3-17 gtable_0.3.0 [53] processx_3.7.0 ensembldb_2.20.2 [55] rlang_1.0.4 utils_4.2.0 [57] methods_4.2.0 genefilter_1.78.0 [59] splines_4.2.0 rtracklayer_1.56.1 [61] lazyeval_0.2.2 DSS_2.44.0 [63] GEOquery_2.64.2 dichromat_2.0-0.1 [65] checkmate_2.1.0 BiocManager_1.30.18 [67] yaml_2.3.5 GenomicFeatures_1.48.3 [69] backports_1.4.1 httpuv_1.6.5 [71] base_4.2.0 Hmisc_4.7-0 [73] usethis_2.1.6 tools_4.2.0 [75] nor1mix_1.3-0 ggplot2_3.3.6 [77] ellipsis_0.3.2 RColorBrewer_1.1-3 [79] BiocGenerics_0.42.0 siggenes_1.70.0 [81] sessioninfo_1.2.2 Rcpp_1.0.8.3 [83] plyr_1.8.7 base64enc_0.1-3 [85] sparseMatrixStats_1.8.0 progress_1.2.2 [87] zlibbioc_1.42.0 purrr_0.3.4 [89] RCurl_1.98-1.7 ps_1.7.1 [91] prettyunits_1.1.1 IlluminaHumanMethylationEPICmanifest_1.0.0 [93] rpart_4.1.16 openssl_2.0.2 [95] deldir_1.0-6 urlchecker_1.0.1 [97] bumphunter_1.38.0 S4Vectors_0.34.0 [99] SummarizedExperiment_1.26.1 cluster_2.1.3 [101] fs_1.5.2 magrittr_2.0.3 [103] data.table_1.14.2 ProtGenerics_1.28.0 [105] IlluminaHumanMethylationEPICanno.ilm10b4.hg19_0.6.0 matrixStats_0.62.0 [107] pkgload_1.3.0 missMethyl_1.30.0 [109] mime_0.12 hms_1.1.1 [111] xtable_1.8-4 XML_3.99-0.10 [113] jpeg_0.1-9 mclust_5.4.10 [115] IRanges_2.30.0 gridExtra_2.3 [117] compiler_4.2.0 biomaRt_2.52.0 [119] minfi_1.42.0 tibble_3.1.7 [121] crayon_1.5.1 R.oo_1.25.0 [123] htmltools_0.5.2 later_1.3.0 [125] tzdb_0.3.0 Formula_1.2-4 [127] tidyr_1.2.0 DBI_1.1.3 [129] ExperimentHub_2.4.0 dbplyr_2.2.1 [131] MASS_7.3-57 DMRcate_2.10.0 [133] rappdirs_0.3.3 Matrix_1.4-1 [135] readr_2.1.2 permute_0.9-7 [137] cli_3.3.0 quadprog_1.5-8 [139] datasets_4.2.0 grDevices_4.2.0 [141] R.methodsS3_1.8.2 parallel_4.2.0 [143] Gviz_1.40.1 GenomicRanges_1.48.0 [145] pkgconfig_2.0.3 GenomicAlignments_1.32.1 [147] IlluminaHumanMethylation450kmanifest_0.4.0 foreign_0.8-82 [149] xml2_1.3.3 foreach_1.5.2 [151] annotate_1.74.0 rngtools_1.5.2 [153] multtest_2.52.0 beanplot_1.3.1 [155] XVector_0.36.0 doRNG_1.8.2 [157] scrime_1.3.5 graphics_4.2.0 [159] callr_3.7.1 stringr_1.4.0 [161] VariantAnnotation_1.42.1 digest_0.6.29 [163] Biostrings_2.64.0 base64_2.0 [165] htmlTable_2.4.1 edgeR_3.38.1 [167] DelayedMatrixStats_1.18.0 restfulr_0.0.15 [169] curl_4.3.2 shiny_1.7.1 [171] Rsamtools_2.12.0 gtools_3.9.2.2 [173] rjson_0.2.21 lifecycle_1.0.1 [175] nlme_3.1-157 Rhdf5lib_1.18.2 [177] askpass_1.1 limma_3.52.2 [179] BSgenome_1.64.0 fansi_1.0.3 [181] pillar_1.7.0 lattice_0.20-45 [183] pkgbuild_1.3.1 KEGGREST_1.36.2 [185] fastmap_1.1.0 httr_1.4.3 [187] survival_3.2-13 remotes_2.4.2 [189] interactiveDisplayBase_1.34.0 glue_1.6.2 [191] png_0.1-7 iterators_1.0.14 [193] BiocVersion_3.15.2 bit_4.0.4 [195] profvis_0.3.7 stringi_1.7.6 [197] HDF5Array_1.24.1 blob_1.2.3 [199] AnnotationHub_3.4.0 org.Hs.eg.db_3.15.0 [201] latticeExtra_0.6-30 memoise_2.0.1 [203] dplyr_1.0.9
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Hi Lucas,
It still does not work for EH1136, following is the output for ExperimentalHub cache and proxy. (When I select EH166 instead of EH1136, it is working.
> ExperimentHub(cache=getExperimentHubOption("CACHE"), proxy=getExperimentHubOption("PROXY"))
snapshotDate(): 2022-04-26
ExperimentHub with 6315 records
# snapshotDate(): 2022-04-26
# $dataprovider: Eli and Edythe L. Broad Institute of Harvard and MIT, NCBI, Jonathan Griffiths, NA, GEO, The Ta...
# $species: Homo sapiens, Mus musculus, Saccharomyces cerevisiae, human gut metagenome, Rattus norvegicus, Sacch...
# $rdataclass: SummarizedExperiment, ExpressionSet, matrix, character, list, DFrame, RaggedExperiment, data.fram...
# additional mcols(): taxonomyid, genome, description, coordinate_1_based, maintainer, rdatadateadded,
# preparerclass, tags, rdatapath, sourceurl, sourcetype
# retrieve records with, e.g., 'object[["EH1"]]'
title
EH1 | RNA-Sequencing and clinical data for 7706 tumor samples from The Cancer Genome Atlas
EH166 | ERR188297
EH167 | ERR188088
EH168 | ERR188204
EH169 | ERR188317
... ...
EH7538 | Visium_humanDLPFC
EH7539 | Visium_mouseCoronal
EH7540 | seqFISH_mouseEmbryo
EH7541 | ST_mouseOB
EH7542 | SlideSeqV2_mouseHPC
Hi @Amarinder,
It seems like a problem with the Cache option.
Please refer to this vignette https://bioconductor.org/packages/devel/bioc/vignettes/AnnotationHub/inst/doc/TroubleshootingTheCache.html#cannot-retrieve-resource
Use the different options progressively, and use the removeCache as the last option. If the problem persists, we may want to loop in the Bioconductor team that are the developers of ExperimentHub.
Below is a snapshot of what you should be able to retrieve.
Best, Lucas
@.***
Lucas
From: Amarinder @.> Sent: Sunday, August 7, 2022 8:01 PM To: immunomethylomics/FlowSorted.Blood.EPIC @.> Cc: Lucas A. Salas @.>; Comment @.> Subject: Re: [immunomethylomics/FlowSorted.Blood.EPIC] Error failed to load with libraryDataGet (Issue #9)
Hi Lucas,
It still does not work for EH1136, following is the output for ExperimentalHub cache and proxy. (When I select EH166 instead of EH1136, it is working.
ExperimentHub(cache=getExperimentHubOption("CACHE"), proxy=getExperimentHubOption("PROXY")) snapshotDate(): 2022-04-26 ExperimentHub with 6315 records
snapshotDate(): 2022-04-26 $dataprovider: Eli and Edythe L. Broad Institute of Harvard and MIT, NCBI, Jonathan Griffiths, NA, GEO, The Ta... $species: Homo sapiens, Mus musculus, Saccharomyces cerevisiae, human gut metagenome, Rattus norvegicus, Sacch... $rdataclass: SummarizedExperiment, ExpressionSet, matrix, character, list, DFrame, RaggedExperiment, data.fram... additional mcols(): taxonomyid, genome, description, coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags, rdatapath, sourceurl, sourcetype retrieve records with, e.g., 'object[["EH1"]]'
title
EH1 | RNA-Sequencing and clinical data for 7706 tumor samples from The Cancer Genome Atlas EH166 | ERR188297 EH167 | ERR188088 EH168 | ERR188204 EH169 | ERR188317 ... ... EH7538 | Visium_humanDLPFC EH7539 | Visium_mouseCoronal EH7540 | seqFISH_mouseEmbryo EH7541 | ST_mouseOB EH7542 | SlideSeqV2_mouseHPC
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Hi Author, I am getting the following error with the function libraryDataGet