The Miniprep template in OTProto causes contamination of neutralisation buffer and wash buffer 2 reagent stocks.
This is because 400 uL is required, which is split into two transfer events when the P300 pipette is used (300 uL, and then 100 uL). The OT2 by default doesn't get a new tip for the second transfer, so residual sample from the first transfer contaminates the reagent stock.
The Miniprep template in OTProto causes contamination of neutralisation buffer and wash buffer 2 reagent stocks.
This is because 400 uL is required, which is split into two transfer events when the P300 pipette is used (300 uL, and then 100 uL). The OT2 by default doesn't get a new tip for the second transfer, so residual sample from the first transfer contaminates the reagent stock.