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intbio-ncl / BiomationScripterLib

A Python library to assist with scripting bio-automation protocols
MIT License
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OT-2 miniprep template causes contamination #1

Closed Brad0440 closed 3 years ago

Brad0440 commented 3 years ago

The Miniprep template in OTProto causes contamination of neutralisation buffer and wash buffer 2 reagent stocks.

This is because 400 uL is required, which is split into two transfer events when the P300 pipette is used (300 uL, and then 100 uL). The OT2 by default doesn't get a new tip for the second transfer, so residual sample from the first transfer contaminates the reagent stock.

Brad0440 commented 3 years ago

This issue is now fixed: https://github.com/intbio-ncl/BiomationScripter/commit/7beb2f21410a46f865d091c43efb7125f068b04e

The transfer is now completed in 2x200 uL transfers with new tips for each transfer.