Unequal lengths in sequence and overlap file

isovic / racon

Ultrafast consensus module for raw de novo genome assembly of long uncorrected reads. http://genome.cshlp.org/content/early/2017/01/18/gr.214270.116 Note: This was the original repository which will no longer be officially maintained. Please use the new official repository here:

https://github.com/lbcb-sci/racon

MIT License

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Unequal lengths in sequence and overlap file #181

Open marco91sol opened 3 years ago

marco91sol commented 3 years ago

Hi,

I have installed racon 1.4.20 version through conda.

Firstly, I used minimap2 for mapping my corrected PacBio reads vs genome assembly. Then I ran racon with the following parameters:

racon -m 8 -x -6 -g -8 -w 500 -t 64 pacbio_reads.fasta mapping.sam de_novo_assembly.fa > racon.fasta.

It gives me the following mistake:

How can I solve it?

Thanks for your help!

Br, Marco

rvaser commented 3 years ago

Hi Marco, how did you run minimap2?

Best regards, Robert