Open naahraissa opened 2 years ago
i used your script to generate a single file from the paired end reads as input for my sequence file
but sill not working
If you want a SAM file please run minimap2 -t 64 -a consensus.fasta ${reads} > illumina.sam
(note the -a).
Hi Rvaser,
i have been very curious comparing racon with hypo polish. Hypo work well but i keep getting this problem:
[racon::Polisher::initialize] loaded target sequences 8.372787 s [racon::Polisher::initialize] loaded sequences 2478.189205 s terminate called after throwing an instance of 'std::invalid_argument' what(): [bioparser::SamParser] error: invalid file format Aborted (core dumped),
troubleshooting with raised issues doesn't help me:
please can you help me with some ideas
this is by command: i am using illumina paired end reads
minimap2 -t 64 consensus.fasta ${reads} > illumina.sam
racon -t 48 CsM2.fastq illumina.sam draft.fa > polished_Male3.racon.fa
Alternatively i tried overalapping the reads with generate and use a paf. file still it didn't work,
minimap2 -x ava-pb -t 8 reads.fa reads.fq > raw_hifiam.paf
Please i need your help. thanks,
Raissa
Originally posted by @naahraissa in https://github.com/isovic/racon/issues/99#issuecomment-1130394489