isovic / racon

Ultrafast consensus module for raw de novo genome assembly of long uncorrected reads. http://genome.cshlp.org/content/early/2017/01/18/gr.214270.116 Note: This was the original repository which will no longer be officially maintained. Please use the new official repository here:
https://github.com/lbcb-sci/racon
MIT License
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Racon error: empty overlap set! #230

Closed lacassee closed 1 year ago

lacassee commented 1 year ago

Hi, I am try to run Racon on nanopore sequenced and porechop trimmed data (1580.fq.gz) with my reference genome (1580.fa) and my minimap2 alignement (1580.bam). minimap2 alignement was genereted with this commande : minimap2 -a ./ref_genome/1580.fa ./3-trimmed_file/1580.fq.gz -o ./5-alignement/1580.sam

Here is my unsuccessful Racon command : racon -t 4 -m 8 -x -6 -g -8 -w 500 ../ref_genome/1580.fa ../5-alignement/1580.sam ../3-trimmed_file/1580.fq.gz

Thank you very much.