Ultrafast consensus module for raw de novo genome assembly of long uncorrected reads. http://genome.cshlp.org/content/early/2017/01/18/gr.214270.116 Note: This was the original repository which will no longer be officially maintained. Please use the new official repository here:
I have long cDNA reads which I want to use as input for genome polishing, expecting that they will only fix exons across the draft reference. Have you tested racon with cDNA alignments before?
I was wondering about how racon deals with "N" operators (CIGAR) of SAM/PAF alignments, as these are suggested to represent introns on mRNA-to-genome alignment. With this in knowledge, do you think it is capable to differentiate between real introns (which are not intended to be removed) and insertions on draft's exons (which should be polished)?
Hi!
I have long cDNA reads which I want to use as input for genome polishing, expecting that they will only fix exons across the draft reference. Have you tested racon with cDNA alignments before?
I was wondering about how racon deals with "N" operators (CIGAR) of SAM/PAF alignments, as these are suggested to represent introns on mRNA-to-genome alignment. With this in knowledge, do you think it is capable to differentiate between real introns (which are not intended to be removed) and insertions on draft's exons (which should be polished)?