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jaleesr / BITFAM

BITFAM is a Bayesian approach and platform to infer transcription factor activities within individual cells using single cell RNA-sequencing data. Please see Gao S et al., Genome Research (2021) https://genome.cshlp.org/content/31/7/1296 for details.
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tiss@data #1

Closed Malarkey73 closed 2 years ago

Malarkey73 commented 2 years ago

Hi I couldn't get this to work. Error is Error in Seurat::FindVariableFeatures(tiss@data) : object 'tiss' not found

BITFAM code contain the line variable_genes <- Seurat::FindVariableFeatures(tiss@data)

shouldn't that just be ? variable_genes <- Seurat::FindVariableFeatures(data)

jaleesr commented 2 years ago

Hi,

Thank you for using our tool! Yes, you are right. It should be data in the function. The "data" here should be a seurat object. If you are using raw data as input, please run BITFAM_preprocess first.

jaleesr commented 2 years ago

@Malarkey73 Please let us know if your issue is resolved. If not, we will gladly provide more suggestions.