jayman1466 / iDOG

MIT License
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Requesting detailed documentation #1

Open drofish6390 opened 1 year ago

drofish6390 commented 1 year ago

Hi Jayman,

New to coding of any kind, and is trying to use iDOG for heterologous expression. I have a pool of sequence variants and the web interface does not support batch processing as far as I know. So when I try to use the command interface I ran into an issue and could not find a solution in the documentation.

Description as follows: I'm using a MacOS 12.5.1 with an intel processor. Environment was created using Anaconda with: python (version 3.9.17) python packages: biopython (version 1.7.9) pillow (version 9.2.0) Vienna RNA Package (version 2.5.1)

The iDOG scripts were downloaded and I cd to the iDOG-master folder. where I get "(SGE-CAD) drofish@Ls-MacBook-Pro iDOG-master %"

To run my sequence, this is what I input:

python codon_opt.py codon_opt(orf_aa = "my_aa_sequence", target_tir = "50000")

and I always get the error message "zsh: unknown sort specifier"

I tried troubleshooting by checking the documentation, but couldn't find information on installation or how to run the functions. Did I use the correct commands or I did do something wrong during installation? I'm not sure if you are still actively monitoring and maintaining this but I gotta try. Any detailed information is appreciated.

Thanks

jayman1466 commented 1 year ago

Hi there, Sorry you seem to be getting this shell error. I haven't run into it before. To try to get to the root cause, could you try to run the core script codon_opt(orf_aa = "", target_tir = "") with either int_RBS = False, terminators = False, or detailed_output = False

Wondering is one of these is the issue.

On Thu, Oct 20, 2022 at 2:17 AM leipan @.***> wrote:

Hi Jayman,

New to coding of any kind, and is trying to use iDOG for heterologous expression. I have a pool of sequence variants and the web interface does not support batch processing as far as I know. So when I try to use the command interface I ran into an issue and could not find a solution in the documentation.

Description as follows: I'm using a MacOS 12.5.1 with an intel processor. Environment was created using Anaconda with: python (version 3.9.17) python packages: biopython (version 1.7.9) pillow (version 9.2.0) Vienna RNA Package (version 2.5.1)

The iDOG scripts were downloaded and I cd to the iDOG-master folder. where I get "(SGE-CAD) @.*** iDOG-master %"

To run my sequence, this is what I input:

python codon_opt.py codon_opt(orf_aa = "my_aa_sequence", target_tir = "50000")

and I always get the error message "zsh: unknown sort specifier"

I tried troubleshooting by checking the documentation, but couldn't find information on installation or how to run the functions. Did I use the correct commands or I did do something wrong during installation? I'm not sure if you are still actively monitoring and maintaining this but I gotta try. Any detailed information is appreciated.

Thanks

— Reply to this email directly, view it on GitHub https://github.com/jayman1466/iDOG/issues/1, or unsubscribe https://github.com/notifications/unsubscribe-auth/AADRSF6CGB77APWLUNP3LGDWEDPWLANCNFSM6AAAAAARJZOEVI . You are receiving this because you are subscribed to this thread.Message ID: @.***>

drofish6390 commented 1 year ago

Hi Jayman,

Thank you very much for replying. Really appreciate your time. I've tried your suggestions (in several ways to avoid confusion). See attached for details.

Screen Shot 2022-10-24 at 18 48 23

Screen Shot 2022-10-24 at 18 52 40

Screen Shot 2022-10-24 at 18 52 54

Screen Shot 2022-10-24 at 18 59 25

As far as I can understand, these still return the same error message. I wonder if I did something wrong in using the commands. Maybe it has to be the specific versions of the packages? Screen Shot 2022-10-24 at 18 48 23 Screen Shot 2022-10-24 at 18 52 40 Screen Shot 2022-10-24 at 18 52 54 Screen Shot 2022-10-24 at 18 59 25

Anyway, thank you very much.