jbloomlab / SARS2-mut-fitness

Observed substitution counts of SARS-CoV-2 compared to those expected under the mutation rates
MIT License
19 stars 5 forks source link

include estimates for ORF9b #22

Closed jbloom closed 1 year ago

jbloom commented 1 year ago

Include fitness estimates for ORF9b, which overlaps in reading frame with N. See #21.

This generalizes the method for handling overlapping reading frames.

The GTF is now edited by a new rule after download to add ORF9b.

Numerical results for all sites change very slightly as this slightly changes the four-fold synonymous site classifications which leads to very slight shifts in the four-fold degenerate site mutation rate estimates that propagate through the analysis. However, these shifts are basically imperceptible unless you look out to several decimal points.

There is now a ORF9b heat map and it is included in the gene distribution plots.