Running error at step2 by low_complexity_filter.pl script

[bioteksampath]

Hi, could me help me out. while running the Mite Hunter step2, im getting these errors,

" Use of uninitialized value $Seq in split at /........./low_complexity_filter.pl line 70. " line 70 script is: @BPs = split(//, $Seq);

Illegal division by zero at /.................../low_complexity_filter.pl line 86. line 86 script is: if(($N_Num / $Len < $Per)&&($Max_Conti_Len < $MaxLen)) { $Undusted_Names{$Name} = 1;

I have no idea about these errors..so could u?

Thanks in advance sam

[hyphaltip]

Looks like a file is Empty or not right format. Can you include sample of the input data used? On Fri, Nov 25, 2016 at 1:37 PM bioteksampath notifications@github.com wrote:

Hi, could me help me out. while running the Mite Hunter step2, im getting this errors,

" Use of uninitialized value $Seq in split at /........./ low_complexity_filter.pl line 70. " line 70 script is: @BPs https://github.com/BPs = split(//, $Seq);

Illegal division by zero at /.................../low_complexity_filter.pl line 86. line 70 script is: if(($N_Num / $Len < $Per)&&($Max_Conti_Len < $MaxLen)) { $Undusted_Names{$Name} = 1;

I have no idea about these errors..so could u?

Thanks in advance sam

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[bioteksampath]

Hi I used the common 500 Mb fasta file seq (since fasta format is not supported uploding here, i uploaded txt format) thanks

seq1.txt

[bioteksampath]

Hi.... Found the problem and solution. It caused by the supporting software (mdust) and the its proper pathing of that at line 21 : system("mdust $Input > $Input.dusted\n"); mdust -> replaced with proper path.