ThruPlex library prep places a UMI as the first 6 nt and then a piece of an adapter that will contain all or part of TCAGTAGCTCA. Right now attempting to trim using that sequence will remove almost all sequence instead of just the adapter part. I found that preprocessing ThruPlex reads to capture the UMI's first is required.
ThruPlex library prep places a UMI as the first 6 nt and then a piece of an adapter that will contain all or part of TCAGTAGCTCA. Right now attempting to trim using that sequence will remove almost all sequence instead of just the adapter part. I found that preprocessing ThruPlex reads to capture the UMI's first is required.