I would like to demultiplex Illumina sequences of the ITS2 region. The barcodes are in the head of the sequence and I have two separated files gITS7 which is forward and ITS4 which is the reverse. Could you please tell me how to deal with two barcodes files, one containing the forward tags and the other the reverse?
Hi,
I would like to demultiplex Illumina sequences of the ITS2 region. The barcodes are in the head of the sequence and I have two separated files gITS7 which is forward and ITS4 which is the reverse. Could you please tell me how to deal with two barcodes files, one containing the forward tags and the other the reverse?
Thanks a lot!