Closed sarahisherb closed 1 year ago
this software has a weird behaviour!
I found a way to get it working. You need to force it to take the righ perl under the right linux group
sarahi@ip29-mp2 ~]$ conda activate METABOLIC_v4.0
(METABOLIC_v4.0) [sarahi@ip29-mp2 ~]$ newgrp def-ilafores
#a second time
sarahi@ip29-mp2 ~]$ conda activate METABOLIC_v4.0
(METABOLIC_v4.0) [sarahi@ip29-mp2 ~]$ /project/6047190/miniconda3/envs/METABOLIC_v4.0/bin/perl /project/def-ilafores/common/METABOLIC/METABOLIC-G.pl -help
DESCRIPTION
Takes a folder containing genome files to generate a profile of their metablic and biogeochemical functions
USAGE
perl METABOLIC-G.pl -t 40 -m-cutoff 0.75 -in Genome_proteins -kofam-db full -o METABOLIC_out
(import genome proteins by users)
perl METABOLIC-G.pl -t 40 -m-cutoff 0.75 -in-gn Genome_files -kofam-db full -o METABOLIC_out
(import genome sequences by users, we will translate them by prodigal)
perl METABOLIC-G.pl -test true
(use the 5 genomes to test the METABOLIC-G script)
OPTIONS
-t or -cpu [integer] The cpu numbers to run the hmmsearch (default: 20)
-m-cutoff or -module-cutoff [float] The cutoff value to assign the presence of a specific KEGG module (KEGG module step present numbers / KEGG module step total number) (default: 0.75)
-in [string] The folder pf given genome faa files [should also give the genome fasta files and genone gene files if the (meta)genome/(meta)transciptome datasets are included]
-in-gn [string] The folder of given genome fasta files (Prodigal will be used to annotate your genomes)
-kofam-db [string] to use the "small" size or "full" size of KOfam database in METABOLIC (default: 'full')
-p or -prodigal-method [string] "meta" or "single" for prodigal to annotate the orf
-o or -output [string] The METABOLIC output folder (default: current address)
-test [string] The option to test the performance of METABOLIC-G by 5 genomes; "true" or "false" to run the test option. The test option will use 5 CPUs to run the command.
INSTRUCTIONS
GitHub: https://github.com/AnantharamanLab/METABOLIC
OUTPUT
Prodigal-annotated protein and gene files will be given in the input folder.
The METABOLIC result table will be generated.
Biogeochemical pathway diagrams will be generated.
COPYRIGHT
Zhichao Zhou, zczhou2017@gmail.com
Salut, just tried to run METABOLIC-G on ip29. conda activation seemed to work, but I got this warning, and I can't run it basically:
perl METABOLIC-G --help
Can't open perl script "METABOLIC-G.pl": No such file or directory