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bravo-protocols
VWorks protocols and other files for the Agilent NGS Workstations at the Genomics facility at SciLifeLab.
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Add new functionality to TruSeq RNA protocol
#17
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jgruselius
closed
5 years ago
jgruselius
commented
10 years ago
Add centrifugation after mixing sample and reagents
Add tRobot incubation
Add possibility to do final hold
Add sealing of plate before 1–3 and at end (conditionally, if last protocol in runset)
Also changed:
~“With-bead” protocol in adapter ligation (beads are added once, then kept after elution and present in following reactions)~.
Implemented and tested but did not work good.
~Related to above; CA purification is separated into three modules: Bead wash, purification, elution.~
Implemented and tested but did not work good either.
The RoboCut size selection will be swapped out for a regular CA purification.
[x] Part 1: mRNA purification, fragmentation and cDNA synthesis
[x] Part 2: A-tailing, adapter ligation
Also changed: