IOError: [Errno 2] No such file or directory:

[GoogleCodeExporter]

I have been trying to install msatcommander 0.8.2 on Ubuntu 12.04 and 
msatcommander 0.8.2 on Windows XP.

When I run msatcommander on testSequence.fsa with 'Design Primers' selected I 
get the same following error on both OSs:

IOError: [Errno 2] No such file or directory: 
u'/home/administrator/Programs/msatcommander-0_8_2/primer3/gi_42495662_gb_AY5229
99_1__primer.txt'

Repeats are found and reported in both cases and written to 
microsatSearchOutput.csv, and a primer3_temp.txt file is written to the Primer3 
output directory. No other primer files are created.

Has anybody come across this before? Any help is much appreciated. Thanks

Original issue reported on code.google.com by danielja...@gmail.com on 24 Mar 2014 at 3:49

[GoogleCodeExporter]

I'm having the same problem, but with the Windows version. I also had 
experienced memory errors when screening Ion Torrent data (4.5M reads) for 
microsat loci - the program would find repeat regions fine, but would not 
design primers (wouldn't even produce the temp.txt file). 

I tried to get around that problem by filtering the fasta read file to have 
only the top 80 loci and tried to use msatcommander to bulk design primers - 
which is when I started getting the above error. So clearly there are multiple 
issues with the primer design component of the Windows version. 

Original comment by krosc...@gmail.com on 9 Jul 2015 at 12:10