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jianhong / cdQuPath

Codex downstream analysis with QuPath
https://jianhong.github.io/cdQuPath/
GNU General Public License v3.0
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seu <- ScaleData(seu, features = all.markers) #1

Closed vijithaduke closed 2 months ago

vijithaduke commented 3 months ago

seu <- ScaleData(seu, features = all.markers) Warning: No layers found matching search pattern providedError in ScaleData(): ! No layer matching pattern 'data' found. Please run NormalizeData and retry Backtrace:

  1. Seurat::ScaleData(seu, features = all.markers)
  2. Seurat:::ScaleData.Seurat(seu, features = all.markers)
  3. Seurat:::ScaleData.StdAssay(...)

    all.markers <- rownames(seu) seu <- ScaleData(seu, features = all.markers) Warning: No layers found matching search pattern providedError in ScaleData(): ! No layer matching pattern 'data' found. Please run NormalizeData and retry Backtrace:

  4. Seurat::ScaleData(seu, features = all.markers)
  5. Seurat:::ScaleData.Seurat(seu, features = all.markers)
  6. Seurat:::ScaleData.StdAssay(...)
jianhong commented 3 months ago

Please run NormalizeData and retry.

vijithaduke commented 3 months ago

sure, will do. thanks!