jiaxuanleong / SPIRO.Assays

ImageJ macros written to analyse data acquired by SPIRO imaging
BSD 2-Clause "Simplified" License
3 stars 0 forks source link

Update SeedCount.ijm #1

Closed jiaxuanleong closed 5 years ago

jiaxuanleong commented 5 years ago

Original idea: ungerminated seeds have a certain roundness range, and germinated seeds have lower roundness range, that does not overlap with ungerminated. So, direct analysis in Analyze Particles is prone to false positives/negatives if the seeds aren't "perfect" in the expected roundness range. A solution: "Roundness" of each seed is tracked individually, and a drop in roundness is detected and interpreted as germination.