Undefined variable in line 2227

[AlyonaMinina]

Solved by upgrading Image J to the 1.53c version

Root growth macro in the debug mode (flipped preprocessed image stacks for etiolation assay):

• overlay enabled
• rsc tracking disabled

Run on Windows10

Error encounter on the fisrt group of the first plate (see the error log in the text below)

Name Value Memory 3033MB of 48601MB (6%) nImages() 1 getTitle() "A control.tif" maindir (g) "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\" listInmaindir (g) array[4] resultsdir (g) "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\Results\" platedir (g) "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\Results\Root Growth..." listInplatedir (g) array[4] ppdir (g) "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\Results\Preprocessin..." DEBUG (g) 1 freshstart (g) 0 selfaware (g) 0 overlay (g) 1 RSCtracking (g) 0 runRegistration (g) 1 batchsize (g) 350 batched (g) 0 rbatched (g) 1 rNonbatched (g) 2 germdir (g) 0 rootgrowthdir (g) "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\Results\Root Growth\" listInrootgrowthdir (g) array[4] step (g) 4 fullplatearray (g) array[16] fullgrouparray (g) array[16] totalnoOfgroups (g) 16 platesToprocess (g) array[16] groupsToprocess (g) array[16] lengthOfgroupsToprocess (g) 16 resumestep (g) 0 overlaychoice "Enable" RSCtrackingchoice "Disable" listInppdir array[4] groupprocess 0 platename "plate1" groupfolder "A control/" groupdir "D:\Users\Alyona\SPIRO\2021.06.12 WP7 XI. Exp 2 Normal vs -C\Results\Root Growth..." listIngroupdir array[8] groupname "A control" mask "A control masked.tif" roicount 31 roiarray array[31] nS 273 rsc "Root start coordinates" sliceno 1 roino 1 xisp 353.8160 yisp 1689.7816 nr 8463 img "A control.tif" row 0 xUP 0 yUP 0

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Error: Undefined variable in line 2227:

. setPosition ( sliceno ) ;