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jimrperkins / ReadqPCR

Read in qPCR data and store as qPCRBatch
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Import data from ixo file ? #1

Open lmanchon opened 3 years ago

lmanchon commented 3 years ago

-hi,

is there a way to import data directly from ixo file ? and if not: how to convert ixo to another format ?

thank you --

stamats commented 3 years ago

No, it's not possible to import ixo-files directly. The only way I know is open the ixo-file with Roche software and export the data e.g. as csv-file. Best Matthias

lmanchon commented 3 years ago

-ah ok, i'm going to write a little python script to get the Ct values from the ixo file, but in the ixo file i have 2 types of Ct: 'CrossingPoint' values and 'CpAverage' values

and i don't know which are the relevants values to then perform the ddCt analysis. do you have an idea ? thank you --

stamats commented 3 years ago

I would assume CrossingPoint = Ct. CpAverage might include the average CP for some (technical?) replicates. Best Matthias

FeBBBbbb commented 1 week ago

@Imanchon I have the same question as you. May I ask how did you parse the CT value from the ixo file

lmanchon commented 1 week ago

ixo files are big text file, so you can parse them using scripting languages such as python, perl, ruby, php...