Open hahmu opened 7 months ago
Hello,
Thank you very much for your amazing works! I downloaded the GSE124395 and copy your code:
data<-readRDS('GSE124395_Normalhumanliverdata.RData') aizarani_healthy<-CreateSeuratObject(data, project="Aizarani_Healthy", min.cells=3, min.features = 300) VlnPlot(aizarani_healthy, features = c("nFeature_RNA", "nCount_RNA"), ncol = 2) aizarani_healthy[["percent.mt"]] <- PercentageFeatureSet(aizarani_healthy, pattern = "^MT-") aizarani_healthy <- subset(aizarani_healthy, subset = nFeature_RNA > 300 & nFeature_RNA < 2500 & percent.mt < 30)
But while checking with violin plot, all cells have percent.mt = 0. Can you help me to deal with this problem? Thank you very much!
Ha
Hello,
Thank you very much for your amazing works! I downloaded the GSE124395 and copy your code:
Aizarani
data<-readRDS('GSE124395_Normalhumanliverdata.RData') aizarani_healthy<-CreateSeuratObject(data, project="Aizarani_Healthy", min.cells=3, min.features = 300) VlnPlot(aizarani_healthy, features = c("nFeature_RNA", "nCount_RNA"), ncol = 2) aizarani_healthy[["percent.mt"]] <- PercentageFeatureSet(aizarani_healthy, pattern = "^MT-") aizarani_healthy <- subset(aizarani_healthy, subset = nFeature_RNA > 300 & nFeature_RNA < 2500 & percent.mt < 30)
But while checking with violin plot, all cells have percent.mt = 0. Can you help me to deal with this problem? Thank you very much!
Ha