Heatmap function always alphabetically plots the rows of the input matrix

[B-1991-ing]

Hi Zuguang,

It is so strange that the Heatmap function doesn't give me the desired input row order when using the rowAnnotation.

What I want is that it directly plots the matrix, because I already sorted the matrix according to the frequency.

But, what I get now.

It feels like the rowAnnotation function doesn't work at all no matter how to set it. If it works, the row annotation should be ordered by the frequency row annotation labels. I can send you my raw data and code to your email if necessary.

rownames_list <- rownames(d) rownames_list_split <- strsplit(rownames_list, ";") Phylum_names <- sapply(rownames_list_split, function(x) x[1]) rownames(d) <- Phylum_names

group1 = rep(c("Bacteroidota", "Proteobacteria", "Desulfobacterota", "Cyanobacteria", "Chloroflexota", "Acidobacteriota", .....), c(67,47,32,25,24,18,14,10,10,9,8,7,7,5,4,3,3,3,3,3,2,2,2,2,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1)) col_list = list(F = c("Bacteroidota"="#03045e", "Omnitrophota"="#0077b6", ......)) ha2 = rowAnnotation(F = Phylum_names, simple_anno_size = unit(0.3, "cm"), col=col_list, show_annotation_name = FALSE)

The row annotation function works on my other matrixes, but just not on this one. So strange.

Best,

Bing

[jokergoo]

Always make sure the order of annotations and the matrix match.

You can send me the data and code, then I will have a look.

[B-1991-ing]

Thank you very much for your reply.

Could you please give me your email address?