How to set the cutoff of the ratio when making basecalls?

jonathonthill / sangerseqR

git for the Bioconductor package "sangerseqR"

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How to set the cutoff of the ratio when making basecalls? #1

Open yin-huamin opened 2 years ago

yin-huamin commented 2 years ago

Hello, author. Could I asd the appropriate cutoff ratio value when making basecalls? I can't find the reference in any paper and the default is 0.2, but it will get error when alignment to the reference genome. I think if the problem is the strict cutoff. Hope for your answer, thanks!