It looks like the makeBaseCalls() function can ignore the dominant peak when a nucleotide is repeated, especially when the signal does not return to zero between nucleotides. I tried to upload an ab1 file, but it's not supported. Instead, I provided the images of the chromatograms before and after makeBaseCalls(), as well as the R script below
It looks like the
makeBaseCalls()
function can ignore the dominant peak when a nucleotide is repeated, especially when the signal does not return to zero between nucleotides. I tried to upload an ab1 file, but it's not supported. Instead, I provided the images of the chromatograms before and aftermakeBaseCalls()
, as well as the R script below