josephreplogle / guide_calling

Tools for sgRNA calling in direct capture Perturb-seq data
MIT License
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input file 904_3_V3.guide_barcode_reads.txt #9

Open XiaoliMa opened 6 months ago

XiaoliMa commented 6 months ago

Hello,

Could you please let me know where I can get the input files: 904_3_V3.guide_barcode_reads.txt or K562 guide RNA cell identifies (https://www.sciencedirect.com/science/article/pii/S0092867422005979?via%3Dihub#mmc1)?

I cannot find the input files for guide calling and confused to how to prepare it, while the demo already had cell_identities.csv files there (probably is result of guide_calling).

MujeebQadiri commented 1 week ago

Hi Joseph, thank you for putting together this neat tool. I have tried using the sftp from previous closed solutions but it is not working, do you mind sharing an updated link?