Closed TaoCheng98 closed 2 years ago
Dear Chengtao, Thank you for your interest in using GenEra! Technically, the method that is implemented in GenEra can be used to analyze any organism. Nonetheless, the software uses the NCBI NR database by default. The eukaryote genomes on the NR contain a considerable amount of prokaryote contamination, which should not have any effect on phylostratigraphy in eukaryotes (if a matching gene is found in a bacterial contaminant, chances are that the gene will be traced back to LUCA either way). However, this poses a problem for prokaryote phylostratigraphy, as some matching eukaryote sequences might end up being bacterial contamination. What I suggest is for you to use a prokaryote database that can be traced back to the NCBI taxonomy as the default database (e.g., Refseq for prokaryotes) and then feed GenEra with a selected list of high-quality eukaryote proteomes, such as the NCBI landmark genomes (https://blast.ncbi.nlm.nih.gov/smartblast/smartBlast.cgi?CMD=Web&PAGE_TYPE=BlastDocs) by using the -a flag, alongside any other proteomes that are of interest for your analysis. You can always contact me through my email (josue.barrera@tuebingen.mpg.de) if you need any help with setting up the database. Best, Josué.
Hi,
GenEra is so great that I would like to use it to study the genomic phylostratigraphy of Mycobacterium tuberculosis.
GenEra appears to have been designed for use in eukaryotes, but I still wonder if GenEra can be used in prokaryotes?
best.
Chengtao