tmp files error & archaea

[arianccbasile]

Dear GapSeq developers, Thank you for the development of such an interesting source. I have installed it and everything went fine. I tried to run ./gapseq doall toy/myb71.fna.gz and I got the following error.

Checking updates for Bacteria /mnt/data_SSD/gapseq/src/../dat/seq/Bacteria 2020-07-24 16:30:56 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/rev/sequences.tar.gz [193] -> ".listing" [1] 2020-07-24 16:31:22 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/rev/sequences.tar.gz [21329707] -> "sequences.tar.gz" [1] 2020-07-24 16:31:22 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/unrev/sequences.tar.gz [194] -> ".listing" [1] 2020-07-24 16:36:55 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/unrev/sequences.tar.gz [254056885] -> "sequences.tar.gz" [1] 2020-07-24 16:36:56 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/rxn/sequences.tar.gz [193] -> ".listing" [1] 2020-07-24 16:36:57 URL: ftp://ftp.rz.uni-kiel.de/pub/medsystbio/Bacteria/rxn/sequences.tar.gz [734237] -> "sequences.tar.gz" [1] Updated Bacteria reviewed sequences Updated Bacteria unreviewed sequences Updated Bacteria additional reaction sequences Error in get(name, envir = asNamespace(pkg), inherits = FALSE) : oggetto "finalize_filexp" non trovato Calls: readAAStringSet ... .read_XStringSet -> fasta.index -> .finalize_filexp_list -> ::: -> get Esecuzione interrotta cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cp: cannot stat '/tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta': No such file or directory Error in get(name, envir = asNamespace(pkg), inherits = FALSE) : oggetto "finalize_filexp" non trovato Calls: readAAStringSet ... .read_XStringSet -> fasta.index -> .finalize_filexp_list -> ::: -> get Esecuzione interrotta cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cp: cannot stat '/tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta': No such file or directory Error in get(name, envir = asNamespace(pkg), inherits = FALSE) : oggetto "finalize_filexp" non trovato Calls: readAAStringSet ... .read_XStringSet -> fasta.index -> .finalize_filexp_list -> ::: -> get Esecuzione interrotta cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cat: /tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta: No such file or directory cp: cannot stat '/tmp/tmp.DjAQqNaSp8/subunit_tmp.fasta': No such file or directory

Do you have any suggestion on how to solve it? Many thanks in advance.

Arianna Basile

[jotech]

Hi Arianna, hm this looks strange indeed! It seems that something in the subunit detection is not running correctly. Do you have exonerate installed? gapseq needs fastaindex and fastafetch from this package.

[arianccbasile]

Hi, I followed your suggestion and I checked exonerate, but it is properly installed. I have performed additional checks on the other dependencies needed and they seem ok. I was wondering if the problem could be due to lack of space in the "/tmp/" directory where gapseq is trying to save these files (there are a couple of Gb available). Alternatively, is it possible to set the working directory where gapseq is writing these files? Thanks again for your assistance. Arianna

[arianccbasile]

Hi, After a series of trials, I managed to fix all the installation problems, they were mainly related to the sybilSBML that was not properly installed at the beginning. We would like to know if you are planning in the next future to complete the section related to Archaea pathways prediction and implementation in the models. Thanks in advance. Sincerely Arianna

[Waschina]

Dear Arianna, We are currently testing and implementing first features for the reconstruction of archaeal metabolic networks. We aim to add the possibility to reconstruct Archaea models in the next 3-4 weeks and provide an example workflow for Archaea in the gapseq documentation. In the meanwhile, the first step of gapseq, the pathway prediction, works already for Archaea. For instance:

$gapseq find -p all -t Archaea ../genomes/genome.fna.gz

I hope this helps and we will let you know, when more features for Archaea are implemented.

Best wishes Silvio

[arianccbasile]

Dear Silvio, thank you a lot for your help and the useful information. Looking forward to the Archaea universe then :)

Best regards, Arianna

[arianccbasile]

Dear Silvio, any news about the Archaea universe? 🥇

[Waschina]

Dear Arianna, yes, we are still performing some tests for Archaea-specific pathways, but with the latest gapseq development version you can do the following example workflow to reconstruct a metabolic model for an Archaeal genome (in this example xyz.fna.gz):

#!/bin/bash

model="xyz"

# Reaction & pathway prediction
./gapseq find -p all -t Archaea $model.fna.gz

# Transporter prediction
./gapseq find-transport $model.fna.gz

# Draft network reconstruction 
./gapseq draft -r $model-all-Reactions.tbl -t $model-Transporter.tbl -p $model-all-Pathways.tbl -l 100 -b archaea

# Gapfilling
./gapseq fill -m $model-draft.RDS -n [media.csv] -c $model-rxnWeights.RDS -g $model-rxnXgenes.RDS -b 100

For [media.csv] please provide the path to the gapfilling media. In the gapseq directory dat/media/ there are two minimal growth media, that we used in our test cases for Methanogens:

• dat/media/MM_anaerobic_Acetate_H2.csv
• dat/media/MM_anaerobic_CO2_H2.csv

We hope this helps and please let us know if you experience issues.

Best regards Silvio

[jotech]

there is also a tutorial on archaea reconstruction out: https://gapseq.readthedocs.io/en/latest/tutorials/archaea.html

[jotech]

please reopen if still an issue