multiPrime is a mismatch-tolerant minimal primer set design tool for large and diverse sequences (e.g. Virus). Here is a web-based version (test: http://multiPrime.cn))
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multiPrim now allows for easy avoidance of mismatches at any position, making it flexible for experienced users. #4
Based on our results, we have identified critical positions that could further improve the efficiency of candidate primers. If you have a large number of candidate primers and are seeking effective primers, we recommend use Param: coordinate in multiPrime.yaml to gain more effective primers.
Based on our results, we have identified critical positions that could further improve the efficiency of candidate primers. If you have a large number of candidate primers and are seeking effective primers, we recommend use Param: coordinate in multiPrime.yaml to gain more effective primers.