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joybio / multiPrime

multiPrime is a mismatch-tolerant minimal primer set design tool for large and diverse sequences (e.g. Virus). Here is a web-based version (test: http://multiPrime.cn))
MIT License
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Error snakemake --configfile multiPrime.yaml -s multiPrime.py --cores 10 --resources mem_mb=80000 #9

Open yuzh2000 opened 10 months ago

yuzh2000 commented 10 months ago

MissingInputException in line 43 of /home/data/t220349/yuzh/multiPrime_test1/multiPrime/multiPrime.py: Missing input files for rule seq_format: output: yuzh/multiPrime_test1/multiPrime/results/Total_fa/1000.format.fa wildcards: virus=1000 affected files: yuzh/multiPrime_test1/multiPrime/test_data/1000.fa

When I run to this step, an error is reported, I installed it according to the video tutorial, may I ask what the problem is？

joybio commented 10 months ago

@yuzh2000 I observed that the path of your input file differs from the script path. I noticed that your present working directory (pwd) is set to 

/home/data/t220349/yuzh/multiPrime_test1/multiPrime/multiPrime.py

but your input file is located at 

yuzh/multiPrime_test1/multiPrime/test_data/1000.fa.

Modifying the input file path should resolve the issue.

joybio commented 10 months ago

@yuzh2000 By the way, If your input sequence is relatively small, and you're seeking potential degenerate primer pairs, our recent project could be beneficial. We've developed a Windows application multiPrime2-GUI also a website https://multiPrime.cn. We would greatly appreciate your assistance in enhancing and refining this tool.

yuzh2000 commented 10 months ago

@joybio You are really a very nice person! Thank you very much for answering my doubts! Wish you happy every day!😊😀