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joybio / multiPrime2-GUI

multiPrime2-GUI: Designing degenerate primers for maximum coverage with minimal degeneracy.
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Sometimes, multiPrime2-GUI fails to terminate. #1

Open Y-antian opened 10 months ago

Y-antian commented 10 months ago

I attempted to execute multiPrime2 on www.multiprime.cn, but encountered unsuccessful results.

joybio commented 10 months ago

Could you please elucidate the issue you're facing, or could you share your bug reports with me? what do you mean that multiPrime2-GUI fails to terminate?

Y-antian commented 10 months ago

1701574285153

The progress indicator refuses to cease, and upon accessing www.multiPrime.cn, I encounter a blank webpage with no results.

joybio commented 10 months ago

It seems that MultiPrime2-GUI is encountering difficulties in identifying appropriate degenerate primer pairs due to the high diversity in your input. The default parameters may not yield results, so consider adjusting some parameters and attempting the process again. Additionally, employing multiPtime to partition your input into smaller clusters could be beneficial.

It's crucial to recognize that MultiPrime2-GUI is specifically designed for degenerate primer design and it does not serve as an update to multiPrime. If your input is highly diversity, multiPrime may be a more suitable choice for your needs.

B.T.W., I will try to fix this problem. Thank you for bringing this to my attention!