Closed HuynhNPT closed 4 years ago
Thanks for the question. In MACS2, the --broad
option simply lowers the statistical threshold to q< 0.1 and increases the maximum distance between significant sites by a factor of 4.
In Genrich, one can alter the peak-calling parameters: the statistical threshold (-p
/-q
) and the maximum distance (-g
). You can also play with -a
, although keep in mind that a short peak with high significance may have the same AUC as a large broad peak with low significance. Finally, you should consider using the -P
argument to explore the effects of different peak-calling parameters more efficiently.
Thank you so much for your input. This is a good place for me to start optimizing.
I was wondering if it will be an issue if we're calling TF peaks instead of histone modification peaks? In MACS2, there's the option to specify whether we're calling narrow or broad peaks. Therefore, I was wondering if
-a
should be changed when processing TF or HM ChIP-seq. Please advice.