Open thomasveith opened 2 years ago
Hello @thomasveith, let me look into it a little bit, I have not able to reproduce it with a quick attempt. Would you mind providing your session info, im suspecting we have a different version of ggplot ? (output of sessionInfo()
)
Thanks for getting back to me. That is quite possible! Here is my session info.
> sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Mojave 10.14.6
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.1/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] xlsx_0.6.5 sctree_0.0.8.0000 SeuratObject_4.0.2 Seurat_4.0.3
loaded via a namespace (and not attached):
[1] wrapr_2.0.9 sn_2.0.0 plyr_1.8.6 igraph_1.2.6
[5] lazyeval_0.2.2 splines_4.1.1 listenv_0.8.0 scattermore_0.7
[9] GenomeInfoDb_1.28.1 ggplot2_3.3.5 TH.data_1.0-10 digest_0.6.27
[13] htmltools_0.5.1.1 viridis_0.6.1 fansi_0.5.0 magrittr_2.0.1
[17] memoise_2.0.0 tensor_1.5 cluster_2.1.2 ROCR_1.0-11
[21] globals_0.14.0 Biostrings_2.60.2 matrixStats_0.60.0 sandwich_3.0-1
[25] spatstat.sparse_2.0-0 colorspace_2.0-2 rvest_1.0.1 blob_1.2.2
[29] ggrepel_0.9.1 rbibutils_2.2.3 dplyr_1.0.7 libcoin_1.0-9
[33] crayon_1.4.1 RCurl_1.98-1.4 jsonlite_1.7.2 spatstat.data_2.1-0
[37] survival_3.2-12 zoo_1.8-9 ape_5.5 glue_1.4.2
[41] polyclip_1.10-0 gtable_0.3.0 zlibbioc_1.38.0 XVector_0.32.0
[45] leiden_0.3.9 future.apply_1.8.1 BiocGenerics_0.38.0 abind_1.4-5
[49] scales_1.1.1 mvtnorm_1.1-2 DBI_1.1.1 GGally_2.1.2
[53] miniUI_0.1.1.1 Rcpp_1.0.7 isoband_0.2.5 metap_1.4
[57] plotrix_3.8-1 viridisLite_0.4.0 xtable_1.8-4 tmvnsim_1.0-2
[61] reticulate_1.24 spatstat.core_2.3-0 bit_4.0.4 Formula_1.2-4
[65] stats4_4.1.1 htmlwidgets_1.5.3 httr_1.4.2 RColorBrewer_1.1-2
[69] TFisher_0.2.0 ellipsis_0.3.2 ica_1.0-2 farver_2.1.0
[73] rJava_1.0-4 pkgconfig_2.0.3 reshape_0.8.8 uwot_0.1.10
[77] deldir_1.0-6 utf8_1.2.2 labeling_0.4.2 tidyselect_1.1.1
[81] rlang_0.4.11 reshape2_1.4.4 later_1.3.0 AnnotationDbi_1.54.1
[85] munsell_0.5.0 tools_4.1.1 cachem_1.0.5 generics_0.1.0
[89] RSQLite_2.2.7 ranger_0.13.1 mathjaxr_1.4-0 ggridges_0.5.3
[93] stringr_1.4.0 fastmap_1.1.0 goftest_1.2-2 bit64_4.0.5
[97] fitdistrplus_1.1-5 purrr_0.3.4 RANN_2.6.1 KEGGREST_1.32.0
[101] pbapply_1.4-3 future_1.21.0 nlme_3.1-152 mime_0.11
[105] xml2_1.3.2 compiler_4.1.1 rstudioapi_0.13 plotly_4.9.4.1
[109] png_0.1-7 spatstat.utils_2.2-0 tibble_3.1.3 stringi_1.7.3
[113] lattice_0.20-44 Matrix_1.3-4 multtest_2.48.0 vctrs_0.3.8
[117] mutoss_0.1-12 pillar_1.6.2 lifecycle_1.0.0 spatstat.geom_2.4-0
[121] Rdpack_2.1.2 lmtest_0.9-38 RcppAnnoy_0.0.19 data.table_1.14.0
[125] cowplot_1.1.1 bitops_1.0-7 irlba_2.3.3 httpuv_1.6.1
[129] patchwork_1.1.1 R6_2.5.0 promises_1.2.0.1 KernSmooth_2.23-20
[133] gridExtra_2.3 IRanges_2.26.0 parallelly_1.27.0 codetools_0.2-18
[137] MASS_7.3-54 assertthat_0.2.1 xlsxjars_0.6.1 withr_2.4.2
[141] sctransform_0.3.2 mnormt_2.0.2 multcomp_1.4-17 S4Vectors_0.30.0
[145] GenomeInfoDbData_1.2.6 mgcv_1.8-36 parallel_4.1.1 grid_4.1.1
[149] rpart_4.1-15 tidyr_1.1.3 inum_1.0-4 Rtsne_0.15
[153] pbdZMQ_0.3-5 partykit_1.2-15 numDeriv_2016.8-1.1 Biobase_2.52.0
[157] shiny_1.6.0
Hey @thomasveith !
Sorry for the late reply ... Nontheless I think I was able to figure out how to fix it ... Would you mind trying out the fix before I move it to the master branch ... You can install the patched version with the following command
remotes::install_github("jspaezp/sctree", ref="bugfix/multiple_fix_double_legend")
So I'm actually getting a different error now. It seems that, no matter what I choose I get an error like the following:
> plot_gates(X, tree_fit, "15")
Error in plot_gates(X, tree_fit, "15") :
15 is not a terminal node in the provided tree fit, try one of:
7, 8, 9, 10, 11, 13, 14, 15
found the error ... I just screwed up on a logical expression to give a "better" error code ...
MB on not running the tests after adding that message ...
No worries. I really appreciate your help! Is there a new patched version I should install?
This guy should have the "updated" patch version, right now its a work in progress since I am also fixing several other issues caused by changes in the API of some dependencies.
remotes::install_github("jspaezp/sctree", ref="bugfix/multiple_fix_double_legend")
as it is right now, it is working with this example Rmd document https://gist.github.com/jspaezp/93cf300d4aaeb94c410edfefab9c8124
I have a Seurat object, its
active.ident
is afactor
with fourlevels
. When I runDimPlot
orplot_flowstyles
the legend has my four cluster labels displayed correctly. However, when I runplot_gates
I see two legends, one of which shows grey for every cluster.tsneNCIN87.pdf upClosePanelG(2,3).pdf gatingNCIN87.pdf
Here is the code I'm running to produce the gate panels (where
X
is my Seurat object):Any advice? Thanks in advance.