Open PuspenduSardar opened 1 year ago
Hello Try updating to 1.6, several bugs were fixed for that script. Best, J
Thanks, @jtamames.
Do I still need to edit the sqm_annot.pl
script to define the desired parameters? I have SqueezeMeta databases built, is v1.6 compatible with those databases?
Regards, Puspendu
Hello Yes it is, no need to update the databases. Just use configure_nodb.pl.
Hello Try updating to 1.6, several bugs were fixed for that script. Best, J
Hello @jtamames,
I installed the v1.6 and tried it. Now it's throwing up a new type of error (bug) which I never had from the previous version.
sqm_annot.pl -f . -s sample.file -t 86 --nocog
SQM_annot v1.6.0, September 2022 - (c) J. Tamames, F. Puente-Sánchez CNB-CSIC, Madrid, SPAIN
This is part of the SqueezeMeta distribution (https://github.com/jtamames/SqueezeMeta)
Please cite: Tamames & Puente-Sanchez, Frontiers in Microbiology 10.3389 (2019). doi: https://doi.org/10.3389/fmicb.2018.03349
Now I will call SqueezeMeta to do my stuff. Please hold on.
mkdir: cannot create directory ‘/home/ps897/sharing/melanoma/contigs/fun_annot’: File exists
SqueezeMeta v1.6.0, September 2022 - (c) J. Tamames, F. Puente-Sánchez CNB-CSIC, Madrid, SPAIN
Please cite: Tamames & Puente-Sanchez, Frontiers in Microbiology 9, 3349 (2019). doi: https://doi.org/10.3389/fmicb.2018.03349
Run started Mon Oct 10 17:23:07 2022 in sequential mode
1 metagenomes found: sqm_out
--- SAMPLE sqm_out --------
Now creating directories
Reading configuration from /home/ps897/sharing/melanoma/contigs/fun_annot/sqm_out/SqueezeMeta_conf.pl
Directory structure and conf files created. Exiting
Died at /home/ps897/miniconda3/envs/SqueezeMeta_1.6/bin/sqm_annot.pl line 223, <in> line 1.
Here is how my sample.file looks
sqm_out melanoma_nr_prot_clean.faa aa
In fact, the script creates a directory named sqm_out
but then stops.
Could you please let me know how to solve this?
Regards, Puspendu
What is there in the sqm_out/results directory?
Hello,
Thanks.
The sqm_out/results
directory is empty! I have attached the screenshot of the sqm_out
directory and SqueezeMeta_conf.pl
and syslog
(as .txt format).
Many Thanks.
Regards, Puspendu
Where is your aa file stored? Is it in /home/ps897/sharing/melanoma/contigs/fun_annot?
If so, what happens if you try to run the script with:
sqm_annot.pl -f /home/ps897/sharing/melanoma/contigs/fun_annot -s sample.file -t 86 --nocog
Tried!
No luck. Same error.
mkdir: cannot create directory ‘/home/ps897/sharing/melanoma/contigs/fun_annot’: File exists
Hello!
I am using
sqm_annot.pl
(modified as per your suggestion in the clossed-issue #540) to annotate a file with ~40 million predicted ORFs. The script repeatedly crashed, saying error running at line 72 of04.rundiamond.pl
and died at line 223 ofsqm_annot.pl
. I am using SqueezeMeta v1.5.2.I am not sure what is wrong here! Is it a memory/space issue? I am running on 3 TB of free space with 480 GB memory and 86 threads.
Could you please help me with this?
Regards, Puspendu