jtamames / SqueezeMeta

A complete pipeline for metagenomic analysis
GNU General Public License v3.0
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Restarting back step 10 without overwriting the data #600

Closed azam17 closed 1 year ago

azam17 commented 1 year ago

Hi,

I have 30 metagenomes data currently being analyzed using squeezemeta, however, the terminal crashed for some reason and no error was written in the syslog file.

Step 10 crashed after processing 4 out of 30 samples. My question is, can i restart again step 10 and continue from sample 5 onwards?

I tried restarting it by running this command: SqueezeMeta.pl -p sqm_soil --restart -step 10, but it automatically moved to step 11 after detecting the .mapcount file exist in intermediate folder. Below is the output from terminal after the command above.

(SqueezeMeta-1.6) amaliamohdhashim@Amalia-Laboratory:~/MPOB_soil_assembly/squeezemeta$ SqueezeMeta.pl -p sqm_soil --restart -step 10 

SqueezeMeta v1.6.0, September 2022 - (c) J. Tamames, F. Puente-Sánchez CNB-CSIC, Madrid, SPAIN

Please cite: Tamames & Puente-Sanchez, Frontiers in Microbiology 9, 3349 (2019). doi: https://doi.org/10.3389/fmicb.2018.03349

Run started Fri Dec 16 00:02:27 2022 in coassembly mode
30 metagenomes found: D1B D1E D1R D2B D2E D2R D3B D3E D3R D4B D4E D4R D5B D5E D5R H1B H1E H1R H2B H2E H2R H3B H3E H3R H4B H4E H4R H5B H5E H5R

Mapping file /home/amaliamohdhashim/MPOB_soil_assembly/squeezemeta/sqm_soil/intermediate/10.sqm_soil.mapcount already found, skipping step 10
[5 seconds]: STEP11 -> COUNTING TAX ABUNDANCES: 11.mcount.pl

syslog.zip