[0 seconds]: STEP10 -> MAPPING READS: 10.mapsamples.pl
Reading samples from /data/mohammad/full_project/data/00.full_project.samples
Metagenomes found: 45
Reading contig length from /data/mohammad/full_project/intermediate/01.full_project.lon
Reading orf info from /data/mohammad/full_project/results/08.full_project.gff
Mapping with Bowtie2 (Langmead and Salzberg 2012, Nat Methods 9(4), 357-9)
Creating reference from contigs
Working with sample 1: S1
Getting raw reads
Aligning to reference with bowtie
BAM file already found in /data/mohammad/full_project/data/bam/full_project.S1.bam, skipping
Calculating contig coverage
Counting with sqm_counter: Opening 12 threads
11435752 reads counted
10494702 reads counted
11437610 reads counted
9540615 reads counted
12618761 reads counted
11406217 reads counted
10414814 reads counted
11205437 reads counted
11629895 reads counted
9559217 reads counted
10419199 reads counted
10957115 reads counted
Working with sample 2: S2
Getting raw reads
cp: missing destination file operand after '/data/mohammad/full_project/temp/full_project.S2.current_1'
Try 'cp --help' for more information.
Aligning to reference with bowtie
stat: No such file or directory
Warning: Could not open read file "/data/mohammad/full_project/temp/full_project.S2.current_1" for reading; skipping...
Error: No input read files were valid
(ERR): bowtie2-align exited with value 1
An error occurred during mapping with command /data/anaconda3/envs/SqueezeMeta/SqueezeMeta/bin/bowtie2/bowtie2 -x /data/mohammad/full_project/data/full_project.bowtie -1 /data/mohammad/full_project/temp/full_project.S2.current_1 -2 /data/mohammad/full_project/temp/full_project.S2.current_2.gz --quiet -p 12 -S /data/mohammad/full_project/data/bam/full_project.S2.sam --very-sensitive-local! at /data/anaconda3/envs/SqueezeMeta/SqueezeMeta/scripts/10.mapsamples.pl line 267.
Stopping in STEP10 -> 10.mapsamples.pl. Program finished abnormally
If you don't know what went wrong or want further advice, please look for similar issues in https://github.com/jtamames/SqueezeMeta/issues
Feel free to open a new issue if you don't find the answer there. Please add a brief description of the problem and upload the /data/mradzieta/mohammad/full_project/syslog file (zip it first)
Died at /kusers/ancillary/mradzieta/anaconda3/envs/SqueezeMeta/bin/SqueezeMeta.pl line 941.
Having this issue at step 10, seems like it can't find the relevant file for sample 2?
Having this issue at step 10, seems like it can't find the relevant file for sample 2?