Hello, I have two samples of 30x ONT data without any repeats or WGBS testing. My goal is to compare the differential methylation level between these two samples(for instance, in the promoter regions of some genes). I would like to know how I can use the methylation_frequency.tsv result file from the following pipeline for comparison between samples. It appears that this file only contains frequency information for each site without supporting read information. Thanks in advance!
Hello, I have two samples of 30x ONT data without any repeats or WGBS testing. My goal is to compare the differential methylation level between these two samples(for instance, in the promoter regions of some genes). I would like to know how I can use the methylation_frequency.tsv result file from the following pipeline for comparison between samples. It appears that this file only contains frequency information for each site without supporting read information. Thanks in advance!
https://nanopolish.readthedocs.io/en/latest/quickstart_call_methylation.html